Kinetic Characterization of Lipid II-Ala:Alanyl-tRNA Ligase (MurN) from Streptococcus pneumoniae using Semisynthetic Aminoacyl-lipid II Substrates

MurM and MurN are tRNA-dependent ligases that catalyze the addition of the first (l-Ala/l-Ser) and second (l-Ala) amino acid onto lipid II substrates in the biosynthesis of the peptidoglycan layer of Streptococcus pneumoniae. We have previously characterized the first ligase, MurM (Lloyd, A. J., Gil...

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Detalhes bibliográficos
Main Authors: De Pascale, Gianfranco, Lloyd, Adrian J., Schouten, James A., Gilbey, Andrea M., Roper, David I., Dowson, Christopher G., Bugg, Timothy D. H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2008
Assuntos:
Enzyme Catalysis and Regulation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3259876/
https://ncbi.nlm.nih.gov/pubmed/18842590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M805807200
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