Efficient recovery of functionally intact mRNA from agarose gels via transfer to an ion-exchange membrane.
A simple method is described for the efficient recovery of intact mRNA from high resolution agarose gels. Fractionation of RNA is accomplished by gel electrophoresis under denaturing conditions using methylmercuric hydroxide. The RNA in the gel is then transferred electrophoretically to a diethylami...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1983
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6190131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.10.3283 |
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