Efficient recovery of functionally intact mRNA from agarose gels via transfer to an ion-exchange membrane.
A simple method is described for the efficient recovery of intact mRNA from high resolution agarose gels. Fractionation of RNA is accomplished by gel electrophoresis under denaturing conditions using methylmercuric hydroxide. The RNA in the gel is then transferred electrophoretically to a diethylami...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1983
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6190131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.10.3283 |
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