Efficient recovery of functionally intact mRNA from agarose gels via transfer to an ion-exchange membrane.
A simple method is described for the efficient recovery of intact mRNA from high resolution agarose gels. Fractionation of RNA is accomplished by gel electrophoresis under denaturing conditions using methylmercuric hydroxide. The RNA in the gel is then transferred electrophoretically to a diethylami...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1983
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6190131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.10.3283 |
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