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Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells
We describe a microchip designed to quantify the levels of a dozen cytoplasmic and membrane proteins from single cells. We use the platform to assess protein–protein interactions associated with the EGF-receptor-mediated PI3K signaling pathway. Single-cell sensitivity is achieved by isolating a defi...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3258586/ https://ncbi.nlm.nih.gov/pubmed/22203961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1110865109 |
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