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Structural insights to the metal specificity of an archaeal member of the LigD 3′-phosphoesterase DNA repair enzyme family

LigD 3′-phosphoesterase (PE) enzymes perform end-healing reactions at DNA breaks. Here we characterize the 3′-ribonucleoside-resecting activity of Candidatus Korarchaeum PE. CkoPE prefers a single-stranded substrate versus a primer–template. Activity is abolished by vanadate (10 mM), but is less sen...

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Detalhes bibliográficos
Main Authors: Das, Ushati, Smith, Paul, Shuman, Stewart
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3258152/
https://ncbi.nlm.nih.gov/pubmed/21965539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr767
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