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The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane.

RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by reaction with 2-iminothiolane followed by a mild ultraviolet irradiation treatment. After removal of non-reacted protein and partial nuclease digestion of the cross-linked 16S RNA-protein moiety, a number of individual cr...

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Bibliografiske detaljer
Main Authors: Wower, I, Brimacombe, R
Format: Artigo
Sprog:Inglês
Udgivet: 1983
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC325806/
https://ncbi.nlm.nih.gov/pubmed/6338481
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