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A versatile method for the coupling of protein to DNA: synthesis of alpha 2-macroglobulin-DNA conjugates.
We describe a simple, general method to link proteins covalently to DNA. The method uses two reagents, N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine and 2-iminothiolane. The former reacts specifically with nonpaired quanine residues and upon reduction generates a free sulfhydryl group. The latter r...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1983
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC325744/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6188106/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.3.659 |
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