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A versatile method for the coupling of protein to DNA: synthesis of alpha 2-macroglobulin-DNA conjugates.

We describe a simple, general method to link proteins covalently to DNA. The method uses two reagents, N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine and 2-iminothiolane. The former reacts specifically with nonpaired quanine residues and upon reduction generates a free sulfhydryl group. The latter r...

詳細記述

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書誌詳細
主要な著者: Cheng, S, Merlino, G T, Pastan, I H
フォーマット: Artigo
言語:Inglês
出版事項: 1983
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC325744/
https://ncbi.nlm.nih.gov/pubmed/6188106
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