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A versatile method for the coupling of protein to DNA: synthesis of alpha 2-macroglobulin-DNA conjugates.
We describe a simple, general method to link proteins covalently to DNA. The method uses two reagents, N-acetyl-N'-(p-glyoxylylbenzoyl)cystamine and 2-iminothiolane. The former reacts specifically with nonpaired quanine residues and upon reduction generates a free sulfhydryl group. The latter r...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1983
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC325744/ https://ncbi.nlm.nih.gov/pubmed/6188106 |
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