Generation of Stable Transgenic C. elegans Using Microinjection

Transgenic Caenorhabditis elegans can be readily created via microinjection of a DNA plasmid solution into the gonad (1). The plasmid DNA rearranges to form extrachromosomal concatamers that are stably inherited, though not with the same efficiency as actual chromosomes (2). A gene of interest is co...

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Detaylı Bibliyografya
Asıl Yazarlar: Berkowitz, Laura A., Knight, Adam L., Caldwell, Guy A., Caldwell, Kim A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2008
Konular:
Developmental Biology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3253564/
https://ncbi.nlm.nih.gov/pubmed/19066505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/833
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