Generation of Stable Transgenic C. elegans Using Microinjection

Transgenic Caenorhabditis elegans can be readily created via microinjection of a DNA plasmid solution into the gonad (1). The plasmid DNA rearranges to form extrachromosomal concatamers that are stably inherited, though not with the same efficiency as actual chromosomes (2). A gene of interest is co...

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Bibliographic Details
Main Authors: Berkowitz, Laura A., Knight, Adam L., Caldwell, Guy A., Caldwell, Kim A.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2008
Subjects:
Developmental Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3253564/
https://ncbi.nlm.nih.gov/pubmed/19066505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/833
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