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Generation of Stable Transgenic C. elegans Using Microinjection
Transgenic Caenorhabditis elegans can be readily created via microinjection of a DNA plasmid solution into the gonad (1). The plasmid DNA rearranges to form extrachromosomal concatamers that are stably inherited, though not with the same efficiency as actual chromosomes (2). A gene of interest is co...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3253564/ https://ncbi.nlm.nih.gov/pubmed/19066505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/833 |
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