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Pyrimidine-specific chemical reactions useful for DNA sequencing.
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
Tallennettuna:
| Päätekijät: | , |
|---|---|
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
1980
|
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324374/ https://ncbi.nlm.nih.gov/pubmed/7443522 |
| Tagit: |
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