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A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter

We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another p...

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Hlavní autoři: Eishingdrelo, Haifeng, Cai, Jidong, Weissensee, Paul, Sharma, Praveen, Tocci, Michael J, Wright, Paul S
Médium: Artigo
Jazyk:Inglês
Vydáno: Bentham Open 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3242404/
https://ncbi.nlm.nih.gov/pubmed/22207892
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2174/1875397301105010122
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