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A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein–Protein Interactions

The use of fluorescently-tagged proteins in microscopy has become routine, and anti-GFP (Green fluorescent protein) affinity matrices are increasingly used in proteomics protocols. However, some protein–protein interactions assays, such as protein complementation assays (PCA), require recloning of e...

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Bibliografische gegevens
Gepubliceerd in:Int J Mol Sci
Hoofdauteurs: Moustaqil, Mehdi, Bhumkar, Akshay, Gonzalez, Laura, Raoul, Lisa, Hunter, Dominic J. B., Carrive, Pascal, Sierecki, Emma, Gambin, Yann
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MDPI 2017
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5751283/
https://ncbi.nlm.nih.gov/pubmed/29232933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms18122681
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