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Binding of non-substrate nucleotides to a restriction endonuclease: a model for the interaction of bam HI with its recognition sequence.

The kinetic constants of the site-specific endonuclease Bam HI for various substrates were determined and binding of non-substrate nucleotides to the enzyme was studied. Agarose gel assays in combination with an integrated Michaelis-Menten equation were used for the evaluation of data. The turnover...

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Sonraí Bibleagrafaíochta
Main Authors: Hinsch, B, Mayer, H, Kula, M R
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1980
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC324101/
https://ncbi.nlm.nih.gov/pubmed/6160464
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