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Binding of non-substrate nucleotides to a restriction endonuclease: a model for the interaction of bam HI with its recognition sequence.

The kinetic constants of the site-specific endonuclease Bam HI for various substrates were determined and binding of non-substrate nucleotides to the enzyme was studied. Agarose gel assays in combination with an integrated Michaelis-Menten equation were used for the evaluation of data. The turnover...

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Vydáno v:Nucleic Acids Res
Hlavní autoři: Hinsch, B, Mayer, H, Kula, M R
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 1980
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC324101/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6160464/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.11.2547
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