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Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.

Poly(A)-containing RNA from the bovine anterior pituitary has been used as a template for the enzymatic synthesis of double-stranded cDNA. The resulting double-stranded cDNA was inserted into the Pst I site of pBR322 with the oligo(dG)-oligo(dC) tailing technique and subsequently cloned in E. coli c...

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Autores principales: Nilson, J H, Thomason, A R, Horowitz, S, Sasavage, N L, Blenis, J, Albers, R, Salser, W, Rottman, F M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1980
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC324017/
https://ncbi.nlm.nih.gov/pubmed/7001369
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