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Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.
Poly(A)-containing RNA from the bovine anterior pituitary has been used as a template for the enzymatic synthesis of double-stranded cDNA. The resulting double-stranded cDNA was inserted into the Pst I site of pBR322 with the oligo(dG)-oligo(dC) tailing technique and subsequently cloned in E. coli c...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1980
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC324017/ https://ncbi.nlm.nih.gov/pubmed/7001369 |
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