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Preparation procedures of proteins and RNA influence the total reconstitution of 50S subunits from E. coli ribosomes.
A two-step procedure has been described for the total reconstitution of 50S ribosomal subunit from E. coli. RNA and proteins are mixed with stoichiometry of 1:1.2 and incubated at 44 degrees C in 4.0 mM Mg2+ followed by a second incubation at 50 degrees C in 20 mM Mg2+ (Dohme and Nierhaus, J. Mol. B...
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| Autores principales: | , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1980
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323967/ https://ncbi.nlm.nih.gov/pubmed/6160479 |
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