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Preparation procedures of proteins and RNA influence the total reconstitution of 50S subunits from E. coli ribosomes.
A two-step procedure has been described for the total reconstitution of 50S ribosomal subunit from E. coli. RNA and proteins are mixed with stoichiometry of 1:1.2 and incubated at 44 degrees C in 4.0 mM Mg2+ followed by a second incubation at 50 degrees C in 20 mM Mg2+ (Dohme and Nierhaus, J. Mol. B...
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| Udgivet i: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1980
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323967/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6160479/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.5.989 |
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