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Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen
BACKGROUND: Studies of nuclear function in many organisms, especially those with tough cell walls, are limited by lack of availability of simple, economical methods for large-scale preparation of clean, undamaged nuclei. FINDINGS: Here we present a useful method for nuclear isolation from the import...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3235078/ https://ncbi.nlm.nih.gov/pubmed/22088094 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-499 |
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