Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen

BACKGROUND: Studies of nuclear function in many organisms, especially those with tough cell walls, are limited by lack of availability of simple, economical methods for large-scale preparation of clean, undamaged nuclei. FINDINGS: Here we present a useful method for nuclear isolation from the import...

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Bibliografiske detaljer
Main Authors: Givens, Robert M, Mesner, Larry D, Hamlin, Joyce L, Buck, Michael J, Huberman, Joel A
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Technical Note
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3235078/
https://ncbi.nlm.nih.gov/pubmed/22088094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-499
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