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Novel Mechanism of RNA Repair by RtcB via Sequential 2′,3′-Cyclic Phosphodiesterase and 3′-Phosphate/5′-Hydroxyl Ligation Reactions

RtcB enzymes are a newly discovered family of RNA ligases, implicated in tRNA splicing and other RNA repair reactions, that seal broken RNAs with 2′,3′-cyclic phosphate and 5′-OH ends. Parsimony and energetics would suggest a one-step mechanism for RtcB sealing via attack by the O5′ nucleophile on t...

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Autors principals: Tanaka, Naoko, Chakravarty, Anupam K., Maughan, Bill, Shuman, Stewart
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3234866/
https://ncbi.nlm.nih.gov/pubmed/22045815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.302133
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