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Distinct Contributions of Enzymic Functional Groups to the 2′,3′-Cyclic Phosphodiesterase, 3′-Phosphate Guanylylation, and 3′-ppG/5′-OH Ligation Steps of the Escherichia coli RtcB Nucleic Acid Splicing Pathway

Escherichia coli RtcB is a founding member of a family of manganese-dependent RNA repair enzymes that join RNA 2′,3′-cyclic phosphate (RNA>p) or RNA 3′-phosphate (RNAp) ends to 5′-OH RNA ((HO)RNA) ends in a multistep pathway whereby RtcB (i) hydrolyzes RNA>p to RNAp, (ii) transfers GMP from GT...

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Pubblicato in:J Bacteriol
Autori principali: Maughan, William P., Shuman, Stewart
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4859591/
https://ncbi.nlm.nih.gov/pubmed/26858100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00913-15
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