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Cloning of a portion of the chromosomal gene for human erythrocyte alpha-spectrin by using a synthetic gene fragment.

A region of minimal codon degeneracy was selected from the amino acid sequence of the amino-terminal alpha I domain of human erythrocyte spectrin to design a 90-base-pair DNA probe for the screening of a human genomic library. Five complementary oligonucleotides were assembled to form a full-length...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Linnenbach, A J, Speicher, D W, Marchesi, V T, Forget, B G
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323304/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3458204/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.8.2397
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