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Thermostable multicopper oxidase from Thermus thermophilus HB27: crystallization and preliminary X-ray diffraction analysis of apo and holo forms

A thermostable multicopper oxidase from Thermus thermophilus HB27 (Tth-MCO) was successfully crystallized using the sitting-drop and hanging-drop vapour-diffusion methods. Crystallization conditions and preliminary X-ray diffraction data to 1.5 Å resolution obtained using synchrotron radiation at 10...

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Main Authors: Serrano-Posada, Hugo, Valderrama, Brenda, Stojanoff, Vivian, Rudiño-Piñera, Enrique
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3232148/
https://ncbi.nlm.nih.gov/pubmed/22139175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911103805X
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