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Purification, crystallization and preliminary X-ray diffraction study on pyrimidine nucleoside phosphorylase TTHA1771 from Thermus thermophilus HB8

Pyrimidine nucleoside phosphorylase (PYNP) catalyzes the reversible phosphorolysis of pyrimidines in the nucleotide-synthesis salvage pathway. In order to study the structure–thermostability relationship of this enzyme, PYNP from the extreme thermophile Thermus thermophilus HB8 (TTHA1771) has been c...

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Detalhes bibliográficos
Main Authors: Shimizu, Katsumi, Kunishima, Naoki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330216/
https://ncbi.nlm.nih.gov/pubmed/17401202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107009980
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