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Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of thymidylate kinase (TTHA1607) from Thermus thermophilus HB8

Nucleotide biosynthesis plays a key role in cell survival and cell proliferation. Thymidylate kinase is an enzyme that catalyses the conversion of dTMP to dTDP using ATP-Mg(2+) as a phosphoryl-donor group. This enzyme is present at the junction of the de novo and salvage pathways; thus, any inhibito...

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Détails bibliographiques
Auteurs principaux: Chaudhary, Santosh Kumar, Jeyakanthan, Jeyaraman, Sekar, Kanagaraj
Format: Artigo
Langue:Inglês
Publié: International Union of Crystallography 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564610/
https://ncbi.nlm.nih.gov/pubmed/23385749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112050208
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