cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts
The entire genome of tobacco mosaic virus (TMV) was copied into a series of subgenomic cDNA clones. cDNA sequences of the 5′ and 3′ ends of TMV were cloned separately. A synthetic oligonucleotide primer was used to generate a Pst I site at the 5′ terminus, whereas a different primer was used to gene...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323178/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593669/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.6.1832 |
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