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cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts

The entire genome of tobacco mosaic virus (TMV) was copied into a series of subgenomic cDNA clones. cDNA sequences of the 5′ and 3′ ends of TMV were cloned separately. A synthetic oligonucleotide primer was used to generate a Pst I site at the 5′ terminus, whereas a different primer was used to gene...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Dawson, William O., Beck, David L., Knorr, David A., Grantham, George L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323178/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593669/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.6.1832
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