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cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts
The entire genome of tobacco mosaic virus (TMV) was copied into a series of subgenomic cDNA clones. cDNA sequences of the 5′ and 3′ ends of TMV were cloned separately. A synthetic oligonucleotide primer was used to generate a Pst I site at the 5′ terminus, whereas a different primer was used to gene...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323178/ https://ncbi.nlm.nih.gov/pubmed/16593669 |
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