Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.

We have extended the method of transposon mutagenesis to the eukaryote, Saccharomyces cerevisiae. A bacterial transposon containing a selectable yeast gene can be transposed into a cloned fragment of yeast DNA in Escherichia coli, and the transposon insertion can be returned to the yeast genome by h...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Seifert, H S, Chen, E Y, So, M, Heffron, F
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1986
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3003748/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.735
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