Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.
We have extended the method of transposon mutagenesis to the eukaryote, Saccharomyces cerevisiae. A bacterial transposon containing a selectable yeast gene can be transposed into a cloned fragment of yeast DNA in Escherichia coli, and the transposon insertion can be returned to the yeast genome by h...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
1986
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3003748/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.735 |
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