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Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae.

We have extended the method of transposon mutagenesis to the eukaryote, Saccharomyces cerevisiae. A bacterial transposon containing a selectable yeast gene can be transposed into a cloned fragment of yeast DNA in Escherichia coli, and the transposon insertion can be returned to the yeast genome by h...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Seifert, H S, Chen, E Y, So, M, Heffron, F
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322939/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3003748/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.3.735
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