RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings. While high specificity is often achieved, biological requirements sometimes necessitate that primers are placed in suboptimal locations which le...

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Detalhes bibliográficos
Main Authors: Dobosy, Joseph R, Rose, Scott D, Beltz, Kristin R, Rupp, Susan M, Powers, Kristy M, Behlke, Mark A, Walder, Joseph A
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3224242/
https://ncbi.nlm.nih.gov/pubmed/21831278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-11-80
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