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Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR
BACKGROUND: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination potential of sequence variants affecting primer-to-template annealing. However, for accurate quantitative PCR (qPCR) assessment of gene expression in populations with gene polymorphisms, the effects of seq...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2741440/ https://ncbi.nlm.nih.gov/pubmed/19715565 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-75 |
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