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Specific Labeling of Threonine Methyl Groups for NMR Studies of Protein-Nucleic Acid Complexes
Specific (13)C labeling of Thr methyl groups has been accomplished by growth of a standard laboratory strain of Escherichia coli on [2-(13)C] glycerol in the presence of deuterated isoketovalerate, Ile, and Ala. Diversion of label from the Thr biosynthetic pathway is suppressed by including Lys, Met...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2011
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3222749/ https://ncbi.nlm.nih.gov/pubmed/22039762 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201496d |
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