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Exploiting E. coli auxotrophs for leucine, valine, and threonine specific methyl labeling of large proteins for NMR applications

A simple and cost effective method to independently and stereo-specifically incorporate [(1)H,(13)C]-methyls in Leu and Val in proteins is presented. Recombinant proteins for NMR studies are produced using a tailored set of auxotrophic E. coli strains. NMR active isotopes are routed to either Leu or...

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Detalhes bibliográficos
Publicado no:J Biomol NMR
Main Authors: Monneau, Yoan R., Ishida, Yojiro, Rossi, Paolo, Saio, Tomohide, Tzeng, Shiou-Ru, Inouye, Masayori, Kalodimos, Charalampos G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4936824/
https://ncbi.nlm.nih.gov/pubmed/27255761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-016-0041-1
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