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Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro.

Thymine glycols were produced in M13 DNA in a concentration dependent manner by treating the DNA with osmium tetroxide (OsO4). For the formation of urea-containing M13 DNA, OsO4-oxidized DNA was hydrolyzed in alkali (pH 12) to convert the thymine glycols to urea residues. With both thymine glycol- a...

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Autori principali: Ide, H, Kow, Y W, Wallace, S S
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1985
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC322108/
https://ncbi.nlm.nih.gov/pubmed/3906566
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