Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro.
Thymine glycols were produced in M13 DNA in a concentration dependent manner by treating the DNA with osmium tetroxide (OsO4). For the formation of urea-containing M13 DNA, OsO4-oxidized DNA was hydrolyzed in alkali (pH 12) to convert the thymine glycols to urea residues. With both thymine glycol- a...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1985
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322108/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3906566/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.22.8035 |
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