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Thymine glycols and urea residues in M13 DNA constitute replicative blocks in vitro.
Thymine glycols were produced in M13 DNA in a concentration dependent manner by treating the DNA with osmium tetroxide (OsO4). For the formation of urea-containing M13 DNA, OsO4-oxidized DNA was hydrolyzed in alkali (pH 12) to convert the thymine glycols to urea residues. With both thymine glycol- a...
Uloženo v:
| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1985
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| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC322108/ https://ncbi.nlm.nih.gov/pubmed/3906566 |
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