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Rapid transfer of DNA from agarose gels to nylon membranes.
The unique properties of nylon membranes allow for dramatic improvement in the capillary transfer of DNA restriction fragments from agarose gels (Southern blotting). By using 0.4 M NaOH as the transfer solvent following a short pre-treatment of the gel in acid, DNA is depurinated during transfer. Fr...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1985
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| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC322039/ https://ncbi.nlm.nih.gov/pubmed/4059056 |
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