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Rapid transfer of DNA from agarose gels to nylon membranes.
The unique properties of nylon membranes allow for dramatic improvement in the capillary transfer of DNA restriction fragments from agarose gels (Southern blotting). By using 0.4 M NaOH as the transfer solvent following a short pre-treatment of the gel in acid, DNA is depurinated during transfer. Fr...
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| Main Authors: | , |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1985
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC322039/ https://ncbi.nlm.nih.gov/pubmed/4059056 |
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