Interaction of the restriction endonuclease ScaI with its substrates.

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa-...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Kita, K, Hiraoka, N, Kimizuka, F, Obayashi, A, Kojima, H, Takahashi, H, Saito, H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1985
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322019/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2997733/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.19.7015
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