Interaction of the restriction endonuclease ScaI with its substrates.
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa-...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1985
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC322019/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2997733/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.19.7015 |
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