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Interaction of the restriction endonuclease ScaI with its substrates.
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa-...
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| Hauptverfasser: | , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1985
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC322019/ https://ncbi.nlm.nih.gov/pubmed/2997733 |
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