Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions

The current gold standard for the culture of human pluripotent stem cells requires the use of a feeder layer of cells. Here, we develop a spatially defined culture system based on UV/ozone radiation modification of typical cell culture plastics to define a favorable surface environment for human plu...

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Detalhes bibliográficos
Main Authors: Saha, Krishanu, Mei, Ying, Reisterer, Colin M., Pyzocha, Neena Kenton, Yang, Jing, Muffat, Julien, Davies, Martyn C., Alexander, Morgan R., Langer, Robert, Anderson, Daniel G., Jaenisch, Rudolf
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2011
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3219112/
https://ncbi.nlm.nih.gov/pubmed/22065768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1114854108
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