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Post-Translational Modifications and Lipid Binding Profile of Insect Cell-Expressed Full-Length Mammalian Synaptotagmin 1
[Image: see text] Synaptotagmin 1 (Syt1) is a Ca(2+) sensor for SNARE-mediated, Ca(2+)-triggered synaptic vesicle fusion in neurons. It is composed of luminal, transmembrane, linker, and two Ca(2+)-binding (C2) domains. Here we describe expression and purification of full-length mammalian Syt1 in in...
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| Hoofdauteurs: | , , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
American Chemical
Society
2011
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| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3217305/ https://ncbi.nlm.nih.gov/pubmed/21928778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi200998y |
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