UBC and YWHAZ as suitable reference genes for accurate normalisation of gene expression using MCF7, HCT116 and HepG2 cell lines

Relative quantification of in vitro gene expression using real-time PCR requires stably expressed reference gene for normalisation. In this study, total RNA from MCF7, HCT116 and HepG2 cells were extracted and converted to cDNA using commercially available kit, and real-time PCR was then performed t...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Chua, Siang Ling, See Too, Wei Cun, Khoo, Boon Yin, Few, Ling Ling
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Springer Netherlands 2011
Gaiak:
Original Research
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3217073/
https://ncbi.nlm.nih.gov/pubmed/21850463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-011-9383-4
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